Lub cell disruptor yog ib qho kev sim siv ntau tshaj plaws siv los rhuav tshem cov kab mob lom thiab tso tawm cov tshuaj intracellular.Lub hauv paus ntsiab lus ntawm kev ua haujlwm ntawm cell breaker yog ua raws li lub hauv paus ntsiab lus ntawm lub cev tawg thiab kev siv tshuab oscillation, thiab lub hom phiaj ntawm kev tawg ntawm tes yog ua tiav los ntawm kev muab lub zog txaus los rhuav tshem cov qauv ntawm cov hlwb.
Lub hauv paus ntsiab lus ntawm kev ua haujlwm ntawm kev cuam tshuam ntawm tes yuav qhia meej hauv qab no.Cov khoom tseem ceeb ntawm kev cuam tshuam ntawm tes muaj xws li tus tswj ceev, lub tshuab crushing chamber, lub pob crushing thiab cov qauv kav dej, thiab lwm yam. Ntawm lawv, tus tswj ceev yog siv los tswj kev sib hloov ceev ntawm lub crushing chamber, uas yog lub thawv rau khaws cia. cov qauv thiab cov khoom sib tsoo, thiab cov khoom sib tsoo ua rau cov hlwb tawg los ntawm kev sib tsoo nrog cov qauv.Ua ntej siv lub cell disruptor, qhov tsim nyog cuam tshuam nruab nrab yuav tsum tau xaiv ua ntej.Feem ntau siv crushing media yog iav hlaws, hlau hlaws thiab quartz hlaws dai.
Cov kev xav tseem ceeb hauv kev xaiv qhov nruab nrab crushing yog qhov xwm txheej ntawm tus qauv thiab lub hom phiaj ntawm kev sib tsoo.Piv txwv li, rau cov hlwb tsis yooj yim, cov iav me me tuaj yeem siv rau kev cuam tshuam;rau cov hlwb nyuaj dua, cov hlaws nyuaj hlau tuaj yeem xaiv tau.Thaum lub sij hawm crushing txheej txheem, muab cov qauv yuav tsum tau crushed rau hauv lub crushing bin, thiab ntxiv ib tug tsim nyog tus nqi ntawm crushing nruab nrab.Tom qab ntawd, qhov kev sib hloov ceev ntawm lub crushing chamber yog tswj los ntawm tus tswj ceev, kom lub crushing nruab nrab thiab cov qauv muaj kev sib tsoo tshuab tsis tu ncua.Cov kev sib tsoo no tuaj yeem cuam tshuam cov qauv ntawm lub xov tooj ntawm tes los ntawm kev hloov pauv hluav taws xob, cuam tshuam cov cell membranes thiab organelles, thiab tso tawm cov ntaub ntawv intracellular.
Cov txheej txheem ua haujlwm ntawm lub xov tooj ntawm tes cuam tshuam feem ntau suav nrog cov hauv qab no tseem ceeb: kev sib hloov ceev, qhov loj thiab qhov ntom ntom ntawm qhov nruab nrab crushing, crushing lub sij hawm thiab kub.Thawj yog qhov kev sib hloov ceev.Kev xaiv ntawm kev sib hloov ceev yuav tsum tau hloov kho raws li ntau hom ntawm tes thiab cov qauv qauv.
Feem ntau, rau cov hlwb mos, kev sib hloov ceev dua tuaj yeem raug xaiv los ua kom cov zaus ntawm kev sib tsoo thiab yog li cuam tshuam lub hlwb kom zoo dua.Rau cov hlwb txhav, vim lawv muaj zog ntau dua, qhov nrawm nrawm tuaj yeem txo kom txo tau cov qauv cuam tshuam.
Qhov thib ob yog qhov loj thiab ntom ntawm crushing nruab nrab.Qhov loj thiab qhov ntom ntawm qhov nruab nrab crushing yuav cuam tshuam ncaj qha rau cov nyhuv crushing.Cov xov xwm cuam tshuam me me tuaj yeem muab cov ntsiab lus sib tsoo ntau dua, ua kom yooj yim rau kev cuam tshuam cov qauv ntawm tes.Loj crushing xov xwm yuav tsum tau ntev crushing lub sij hawm.
Tsis tas li ntawd, qhov ceev ntawm crushing nruab nrab yuav tseem cuam tshuam lub zog ntawm kev sib tsoo, dhau qhov ntom ntom yuav ua rau muaj kev sib tsoo dhau los ntawm cov qauv.Lub sijhawm cuam tshuam yog qhov tseem ceeb rau kev cuam tshuam ntawm tes.Kev xaiv ntawm lub sijhawm crushing yuav tsum tau txiav txim siab raws li tus qauv qauv thiab cov nyhuv crushing.Feem ntau, lub sijhawm cuam tshuam ntev dua, lub hlwb raug cuam tshuam ntau dua, tab sis nws kuj tuaj yeem ua rau muaj kev puas tsuaj rau lwm qhov ntawm cov qauv.Qhov kawg yog qhov ntsuas kub.Qhov cuam tshuam ntawm qhov kub thiab txias ntawm cell fragmentation tsis tuaj yeem tsis quav ntsej.Kev kub siab heev tuaj yeem ua rau muaj qhov tsis zoo ntawm cov protein thiab nucleic acids hauv cov hlwb, yog li cuam tshuam rau cov nyhuv fragmentation.Yog li ntawd, nws raug nquahu kom ua kev cuam tshuam ntawm tes nyob rau hauv cov xwm txheej cryogenic, uas tuaj yeem txo qis los ntawm kev siv lub chiller lossis ua haujlwm ntawm cov dej khov.
Cell disruptors ua lub luag haujlwm tseem ceeb hauv kev tshawb fawb lom neeg.Los ntawm kev tswj xyuas tsis zoo xws li kev sib hloov ceev, qhov loj thiab qhov ceev ntawm crushing nruab nrab, crushing lub sij hawm thiab kub, npaum crushing ntawm cov hlwb yuav ua tau tiav.Tom qab cov hlwb tawg lawm, ntau hom tshuaj hauv lub hlwb tuaj yeem tau txais, xws li cov proteins, nucleic acids, enzymes, thiab lwm yam, uas muab qhov tseem ceeb rau kev soj ntsuam thiab kev tshawb fawb tom ntej.Nyob rau hauv luv luv, lub cell disruptor yog ib qho tseem ceeb ntawm kev sim ntsuas, thiab nws txoj kev ua hauj lwm yog raws li lub hauv paus ntsiab lus ntawm lub cev tawg thiab mechanical vibration.Kev cuam tshuam zoo ntawm cov hlwb tuaj yeem ua tiav los ntawm kev tswj hwm qhov sib txawv xws li kev sib hloov ceev, qhov loj thiab qhov ceev ntawm kev cuam tshuam nruab nrab, cuam tshuam lub sijhawm thiab kub.Lub cell disruptor yog siv dav, muab kev yooj yim thiab kev txhawb nqa rau cov kws tshawb fawb hauv kev tshawb fawb txog kev tshawb fawb txog biology.
Post lub sij hawm: Sep-06-2023